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Filetype: .docx. Downloads: 60. Language: Urdu. Size: 19.3 MB. Note: · FreeRead – Urdu web tool — available on all major operating systems — which .Expression of subtypes of alpha2, delta and kappa opioid receptors in the rat subfornical organ.
The subfornical organ (SFO) participates in the regulation of fluid and electrolyte homeostasis as well as blood pressure. Previous studies have demonstrated the role of opioid peptides, which exert their effects through the activation of different opioid receptor subtypes (mu, delta and kappa), in the regulation of several functions of the SFO. In the present study, the expression of different opioid receptor subtypes (alpha(2), delta and kappa) in rat SFO was examined using RT-PCR, PCR and immunohistochemistry. RT-PCR results showed the presence of mRNA encoding the three subtypes of opioid receptors in the SFO. Quantitative PCR analysis revealed the presence of delta opioid receptor mRNA, whereas no expression of alpha(2) or kappa opioid receptor mRNA was detected. Using immunohistochemistry for the three opioid receptor subtypes, specific immunoreactivity was found in SFO neurons and neuropils. The subfornical organ receives afferent inputs from many distinct areas, including the medial preoptic area, subfornical organ-anterior part of the area postrema, posterior area of the nucleus of the solitary tract, supraoptic nucleus and periventricular part of the dorsomedial nucleus. These data suggest that the SFO may play a role in fluid and/or electrolyte homeostasis through the activation of opioid receptors, and that the distribution of opioid receptor subtypes in the SFO may reflect the nature of the endogenous opioid system controlling these functions.Q:
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