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Morphological and physiological features of the pulmonary circulation of male Sprague-Dawley rats which were given monocrotaline (MCT) and which developed pulmonary hypertension (PH) were compared to that of a previous cohort of rats which had been given MCT alone. Dogs were given an intravascular injection of MCT (60 mg/Kg) or equal volume of saline (0.15 M/Kg). Thirty minutes after MCT or saline had been given, the dogs were sacrificed by exsanguination. The pulmonary circulations of control and MCT dogs were compared. Twenty-eight days after MCT treatment, blood pressure (B.P.), heart rate (HR), cardiac output (CO) and pulmonary vascular resistance (PVR) were measured in resting and exercise states. Following sacrifice, the lungs were fixed by intratracheal instillation of a 2% glutaraldehyde solution. Resin-embedded tissues were sectioned. Morphological changes were assessed by viewing sections with the use of light microscopy after staining with hematoxylin and eosin. Morphometric analysis was performed on the sections from the 0.1, 0.2 and 0.3 mm arterial segments distal to the heart, and blood vessel number, diameter, intimal thickness and media area were determined by computer. The data indicate that MCT-treated dogs consistently developed PH; they were characterized by an increase in PVR (p less than 0.01) which was associated with an increase in blood vessel number, diameter and intimal thickness and a decrease in medial area (p less than 0.01). When these results are compared to our prior investigation of control dogs, the data indicate that MCT does induce PH in the dog. However, in contrast to the dog, the rat given MCT for 28 days did not develop PH. After 28 days of MCT treatment, blood pressure (B.P.), HR, CO and PVR were measured. Blood vessels that were classed as large, medium and small were isolated from the right ventricle for in vitro study. Pressure-d
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