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Rhizosphere soils are a good medium for the isolation of microorganisms. Calotropis gigantea has been found to have effective rhizospheric bacterial and fungal populations as revealed by the isolation of various actinomycetes and fungi associated with the plant. A specific morphotype, i.e., sclerotia-forming fungi, were isolated from rhizosphere of C. gigantea which could be differentiated from the other actinomycetes and fungi. Morphological characters such as macroscopic and microscopic appearance and staining characters such as CTAB, Congo red and sulphuric acid were used to differentiate between sclerotia and pycnidia. The isolates were tested for sclerotia formation on PDA, G25 (a low-nutrient medium), Czapek and potato dextrose agar. Sclerotia formation ability was tested on Czapek agar under laboratory conditions (28 degrees C, 14 days), in vivo conditions (on stem) and in vitro conditions (on leaf). The isolates not only produced sclerotia on G25 and Czapek agar but also produced sclerotia in the field on C. gigantea. Microscopic studies revealed the mycelial hyphae produced sclerotia in vitro and in vivo on stem of C. gigantea. Scanning electron microscopy (SEM) technique was used to observe the morphology of sclerotia. Different species of fungi were produced in vitro and in vivo in association with the plant. Extensive tests carried out in the laboratory and the in vivo and in vitro tests on different media have confirmed the results obtained for the first time with respect to the fungi associated with plants.(CN) – A Florida-based health care distributor won a decades-long libel battle against the manufacturer of the painkiller Norco after the company claimed the firm defamed it in ads, state and federal court documents show.

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