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In this study, we examined the temporal changes in the structure and morphology of hepatitis E virus (HEV) and bovine enteric adenovirus 3 (BAV3) virus-like particles (VLPs). A structural protein, E3s, of BAV3 was expressed as a VLP in the cells of HEK293-6E cells, and its morphology was observed to change as the VLP matured. For the HEV VLP, as the maturation proceeded, the filaments extended to 16 nm in length and the number of extended particles increased. The filaments exhibited a maximum diameter of 3 nm (30-fold of the single HEV core), which was consistent with the diameter of the mature HEV particle (60 nm in the case of an HEV genome). The filamentous structures were similar in size to the HEV coat protein (p20). The ratio of the extended particles to the total number increased and the measured diameter of the extended particles was consistent with the single
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Download 1 0Dynamics of particles trapped in viscous fluid flows.
The dynamics of a spherically confined inertial Brownian particle moving in a viscous fluid is studied numerically. The particle can be subjected to a homogeneous, radially symmetric background flow with both steady and transient oscillatory flows. The background velocity field is imposed either as boundary conditions to a set of incompressible Navier-Stokes equations for an inviscid fluid or as a set of Navier-Stokes equations for a viscous fluid. Two methods of numerically solving the differential equations are used. The direct method for Navier-Stokes equations includes the Navier slip boundary condition to compute the tangential velocities on the wall. This boundary condition is expected to apply in the case of the background flow field oscillating due to a pressure gradient. With this boundary condition for the Navier-Stokes equations, a set of ordinary differential equations for the motion of the center of mass of the particle can be analytically solved. Theoretical results obtained by this analysis are compared with the results of direct numerical simulations. The effect of the parameters of the flow velocity profile on the dynamics of the particle is investigated.16S ribosomal RNA-encoding gene sequence analysis of Pasteurella species isolated from pigs with systemic infection.
To investigate the incidence of Pasteurella spp. in pigs, 310 samples of blood, bile, brain, heart, liver, lung, kidney, and spleen from clinically healthy slaughtered animals, as well as 120 bone marrow samples from pigs with systemic diseases, were tested for the presence of Pasteurella spp. using a conventional PCR. Pasteurella species were isolated from 46 (15%) of the 310 blood, 19 (6.1%) of the 310 bile, nine (2.9%) of the 310 bone marrow, and one (0.3%) of the 310 brain samples. Based on amplification of the V-beta domain of the 16S rRNA gene, isolates were identified as P. multocida (n = 21), P. trehalosi (n = 9), P. dagmatis (n = 6), P. histicola (n = 5), P. canis (n = 4), P. trehalosi-like (n = 3), P. dagmatis-like (n =
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